Soybean Breeding Array
| "东农豆芯"系列大豆育种芯片由东北农业大学大豆遗传改良团队研发， 包括液相育种芯片（20K）与固相育种芯片（10K）两大产品体系， 覆盖产量、品质、抗病、抗虫等6大育种模块共35个性状。 |
液相育种芯片 — 20K Liquid-phase Chip
靶向测序基因型分型（GBTS）技术，与博瑞迪生物合作研发。 通过对目标位点高深度重测序精准检测基因型，检测成本较进口芯片降低30–50%。
| Type | # | % |
|---|---|---|
| SNP | 21,359 | 99.24 |
| INDEL | 163 | 0.76 |
| Total | 21,522 | 100 |
| Region | # | % |
|---|---|---|
| CDS | 7,747 | 36.00 |
| Promoter | 6,294 | 29.24 |
| Intergenic | 4,752 | 22.08 |
| Intron | 2,258 | 10.49 |
| 3' UTR | 463 | 2.15 |
| 5' UTR | 8 | 0.04 |
| Category | # | % |
|---|---|---|
| Background | 14,039 | 65.23 |
| Functional | 3,953 | 18.37 |
| GWAS | 1,913 | 8.89 |
| Selected | 1,101 | 5.12 |
| QTL | 429 | 1.99 |
| Authenticity | 65 | 0.30 |
| Wild | 22 | 0.10 |
| Transgenic | 10 | 0.05 |
Performance
| Detection Rate | 100% |
| Replicate Consistency | 99.1% – 99.5% (avg 99.2%) |
| Sequencing vs Chip Concordance | 92% – 98% (avg 97%) |
固相育种芯片 — 10K Solid-state Chip
国内首款自主研发的大豆固相育种芯片，与拉索生物合作研发。 基于1700份东北地区大豆核心种质资源开发，单样本成本极低，检测周期仅需3天。
Solid-state Chip BeadArray Technology — 25 mm × 75 mm substrate with microbead array
| Type | # | % |
|---|---|---|
| SNP | 10,640 | 98.45 |
| INDEL | 145 | 1.34 |
| Transgenic | 22 | 0.20 |
| Total | 10,807 | 100 |
| Region | # | % |
|---|---|---|
| Promoter | 3,893 | 36.02 |
| Intergenic | 2,690 | 24.89 |
| CDS | 2,550 | 23.60 |
| Intron | 1,314 | 12.16 |
| 3' UTR | 333 | 3.08 |
| 5' UTR | 5 | 0.05 |
| Category | # | % |
|---|---|---|
| Functional | 3,336 | 30.87 |
| Background | 2,993 | 27.70 |
| GWAS | 2,137 | 19.77 |
| Selected | 1,214 | 11.23 |
| Wild | 638 | 5.90 |
| QTL | 405 | 3.75 |
| Authenticity | 65 | 0.30 |
| Transgenic | 22 | — |
Performance
| Detection Rate | 95.03% – 99.35% (avg 98.05%) |
| Sequencing vs Chip Concordance | 94.53% – 99.34% (avg 98.59%) |
Breeding Module Design — 育种模块化设计
"东农豆芯"围绕大豆育种核心需求建立6大育种模块、共计35个性状， 推动育种从"经验型"向"设计型"转型。
Quality 5
- Oil content
- Protein content
- Nutritional composition
- Isoflavone
- Seed characteristics
Yield 6
- Seed weight
- Plant architecture
- Seed size
- Chlorophyll content
- Pod number
- Leaf senescence
Disease Resistance 5
- Phytophthora root rot
- SMV
- Fusarium root rot
- Sclerotinia blight
- Sudden Death Syndrome
Insect Resistance 6
- SCN
- Aphid
- Pod borer
- Japanese beetle
- Root-knot nematode
- Cutworm / Bollworm
Abiotic Stress 6
- Drought
- Salt
- Flooding
- Heavy metal
- UV-B radiation
- Low phosphorus
Other 6
- Dormancy
- Pod shattering
- Flowering
- Nodulation
- Fertility
- Regeneration
Application Scenarios
🧬
Variety Authenticity
🔬
Transgenic Detection
🌱
Marker-assisted Breeding
📊
Genomic Selection
🔗
QTL Mapping
🧑🌾
Derived Variety Diagnosis